Pristionchus pacificus is an attractive model organism for laboratory studies based on several technical features:
The following tools have been established over the years that allow mechanistic studies in this nematode:
We are permanently improving our methodological platform in the following areas:
Igreja, C., Loschko, T., Schäfer, A., Sharma, R., Quiobe, S. P., Aloshy, E., Witte, H. & Sommer, R. J. (2022): Application of ALFA-tagging in the nematode model organisms Caenorhabditis elegans and Pristionchus pacificus. Cells, 11, 3875.
Wighard, S. S., Athanasouli, M., Witte, H., Rödelsperger, C. & Sommer, R. J. (2022): A new hope: A hermaphrotditic nematode enables analysis of a recent whole genome duplication event. Genome Biology and Evolution, 14, evac169.
Lo, W.-S., Roca, M., Dardiry, M., Mackie, M., Eberhardt, G., Witte, H., Hong, R., Sommer, R. J. & J. W. Lightfoot (2022): Evolution and diversity of TGF-β signalling pathways are linked with novel developmental and behavioural traits. Molecular Biology and Evolution, 39: msac252.
Röseler, W., Collenberg, M., Yoshida, K., Lanz, C., Sommer, R.J. & Rödelsperger, C. (2022): The improved genome of the nematode Parapristionchus giblindavisi provides insights into lineage-specific gene family evolution. G3, 12, jkac215.
Sun, S., Rödelsperger, C. & R. J. Sommer (2021): Single worm transcriptomics isentifies a developmental core network of oscillating genes with deep conservation across nematodes. Genome Research, 31, 1590-1601.
Sun, S., Rödelsperger, C. & R. J. Sommer (2021): Single worm transcriptomics isentifies a developmental core network of oscillating genes with deep conservation across nematodes. Genome Research, 31, 1590-1601.
Athanasouli, M., Witte, H., Weiler, C., Loschko, T., Eberhardt, G., Sommer, R. J. and C. Roedelsperger (2020): Comprataive genomics and community curation further improve gene annotation in te nematode Pristionchus pacificus. BMC Genomics, 21, 708.
Han, Z., Lo, W.-S., Lightfoot, J. W., Witte, H., Sun, S. & R. J. Sommer (2020): Improving trangenesis efficiency and CRISPR-associated tools through codon optimization and native intron addition in nematodes. Genetics, 216, 947-956.
Witte, H., Moreno, E., Rödelsperger, C., Kim, J., Kim, J.-S., Streit A. & Sommer, R. J. (2015): Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus. Dev Genes & Evol., 225, 55-62.
Rödelsperger, C., Neher, R. A., Weller, A., Eberhardt, G., Witte, H., Mayer, W., Dieterich, C. & Sommer, R. J. (2014): Characterization of genetic diversity in the nematode Pristionchus pacificus from population-scale resequencing data. Genetics, 196, 1153-1165.
Schlager, B., Wang, X., Braach, G. & Sommer, R. J. (2009): Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode model Pristionchus pacificus. Genesis, 47, 300-304.
Dieterich, C., Clifton, S. W., Schuster, L. N.,Chinwalla, A., Delehaunty, K., Dinkelacker, I., Fulton, L., Fulton, R., Godfrey, J., Minx, P., Mitreva, M., Roeseler, W., Tian, H., Witte, H., Yang, S. P., Wilson, R. K., Sommer, R. J. (2008): The Pristionchus pacificus genome provides a unique perspective on nematode lifestyle and parasitism. Nature Genetics, 40, 1193-1198.
Tian, H, Schlager, B., Xiao, H. & Sommer, R. J. (2008): Wnt signaling induces vulva development in the nematode Pristionchus pacificus. Current Biology, 18, 142-146.
